Naslov
Fungal-bacterial teams during the colonization of artificial soils

Autori
Steinbach, Annelie ; Giebler, Julia ; Tanuwidjaja, Irina ; Centler, Florian ; Schulz, Stefanie ; Pronk, Geertje ; Harms, Hauke ; Schloter, Michael ; Wick, Lukas Y.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Annual Meeting of the German Priority Programme SPP 1315: Biogeochemical Interfaces in Soil

Mjesto i datum
Jena, Njemačka, 09.10.2013. - 11.10.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Biogeochemical interfaces ; artificial soils ; microbial communities ; co-occurence ; T-RFLP ; F-ARISA

Sažetak
Various soil aging experiments in the priority program indicated that with increasing soil age, the impacts of soil composition, i.e. heterogeneity of biogeochemical interfaces (BGI), and soil maturation become more prominent fostering accordingly matured community compositions and niche formation. Here, we investigated the role of fungi and the interactions with bacteria in four two year aged artificial soils during the degradation of plant litter. The soils contained quartz sand and silt and either montmorillonite or illite or mixtures of montmorillonite and charcoal or illite and ferrihydrite. These mixtures were inoculated with a natural microbial inoculum (water-extracted from a Luvisol) and subsequently aged for two years. Afterwards, they were amended with maize-potato litter (1 % (w/w)) and incubated for 7, 21 and 63 days. The microbial communities were analyzed using quantitative PCR (qPCR) and terminal restriction fragment length polymorphism analysis (T-RFLP) and fungal ribosomal intergenic spacer analysis (F-ARISA), targeting phylogenetic marker genes for bacteria and fungi, respectively. A network modeling approach should subsequently reveal co-colonization of fungi and bacteria in the different soils based on co-occurrence patterns derived from an integrative analysis of the individual fingerprints. After two years of aging no soil type dependent differences of fungal abundance were observed, whereby the addition of plant litter generally increased the ITS gene copy numbers. Likewise, no strong stimulation of fungi was observed over time. In contrast, soil-dependent patterning in the fungal community fingerprinting was observed after two years of aging. Whereas the two illite-containing soils did not differ significantly from each other, indicating only minor influences of ferrihydrite, the addition of charcoal evoked a strong community divergence from soil with only montmorillonite. Moreover, the fungal community in the charcoal-containing soil was more similar to these of illite soils. However, most communities strongly converged after litter amendment. Solely charcoal-containing soil displayed only minor changes in response to the plant material. Bacterial communities also showed soil type-dependent patterns after aging to some extent, but here all communities strongly diverged after litter addition (for details see abstract of Tanuwidjaja et al.). Statistical co-occurrence analyses showed distinct associative and avoidance relationships of fungal and bacterial fragments which were accordingly influenced by soil composition. This indicates significant BGI-specific fungal-bacterial interactions during soil colonization. Shared niche preferences and helper-effects (e.g. preferential dispersal along mycelia (“fungal highways”), hence, might foster soil type dependent community patterns in different soil types.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA