Naslov
Phytopthora spp. isolated from chestnut populations in the Republic of Macedonia

Autori
Risteski, Mihajlo ; Kuzmanovska, Biljana ; Rigling, Daniel ; Ježić, Marin ; Sotirovski, Kiril

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts "Sustainable Forestry - Fact or Fiction?" / - Skopje : Ss. Cyril and Methodius University, 2017, 47-47

ISBN
978-9989-132-19-3

Skup
Sustainable Forestry - Fact or Fiction?

Mjesto i datum
Skopje, Sjeverna Makedonija, 04.10.2017. - 06.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chestnut populations, disease, FLO

Sažetak
Symptoms specific for Phytophthora spp. on sweet chestnut (Castanea sativa) have been previously reported in Macedonia, but have never been confirmed by isolation of cultures or molecular techniques. We surveyed 15 chestnut populations, aged 15 to 70 years, at sites: Skudrinje, Osoj, Knezino, Straza, Recane. Vrutok, Kale, Kalista, Trebenista, Vratnica and Smolari throughout Macedonia. We assessed for presence of collar and root rot and lesions, as well as crown and seedlings symptoms. Over 130 soil and bark samples were collected from both symptomatic and asymptomatic chestnut plants. Using the bait method, and culturing on PARPNH and CMA+ Phytophthora-selective nutritious media, we isolated 71 Phytophthora-like cultures. We extracted DNA from all cultures, and DNA samples were amplified and sequenced using ITS4 and ITS 6 universal Phytophthora primers. Using online Phytophthora database blast, we identified 5 isolates of P. cambivora and 4 of P. cactorurn. We tested the pathogenicity of one random isolate representative for each of the two species, on excised chestnut sticks collected from a single coppice. We tested on 40 sticks per isolate, 20 per two sets of diameters (5-10 mm and 10-15 mm) of the same length (10 cm). The tests pointed out P.cactorum as more pathogenic than P. cambivora, both on thicker and thinner sticks. This is the first report in Macedonia about identifying these species through cultures and partial sequencing and assessment of their pathogenicity.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Šumarstvo

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