Naslov
Viruses in biocontrol: CHV1-affected epigenetic and biochemical changes in Cryphonectria parasitica

Autori
Ježić, Marin ; Nuskern, Lucija ; Tkalec, Mirta ; Liber, Zlatko ; Krstin, Ljiljana ; Katanić, Zorana ; Mlinarec, Jelena ; Ćurković-Perica ; Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts "Sustainable forestry: Fact or Fiction?" / - Skopje : Ss. Cyril and Methodius University, 2017, 24-24

ISBN
978-9989-132-19-3

Skup
Sustainable forestry: Fact or Fiction?

Mjesto i datum
Skopje, Sjeverna Makedonija, 04.10.2017. - 06.10.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biological control, enzyme activity, genome methylation, hypovirulence

Sažetak
Cryphonectria parasitica is a phytopathogenic fungus introduced from Eastern Asia to North America and to Europe, where it causes chestnut blight, a devastating disease of chestnut trees. A hyperparasitic mycovirus, Cryphonectria hypovirus 1 (CHV1), can infect the fungus, causing the change in morphology and virulence of the affected mycelia, indicating physiological changes. In order to elucidate what changes occur after the infection we have transferred six CHV1 strains belonging to three different virus subtypes into three different, but isogenic, C. parasitica isolates. Using methylation-sensitive amplification polymorphism (MSAP) we assessed the methylation pattern changes of the C. parasitica genome following the infection. The most obvious change was the increase of the number and diversity of methylated, hemi-methylated, and total MSAP markers found in infected fungal isolates compared to virus-free controls. The increase in methylation levels correlated well with the CHV1-induced reduction of fungal growth in vitro, indicating that C. parasitica genome methylation upon CHV1 infection, rather than being the defensive mechanism of the fungus, is more likely to be the virulence determinant of the virus. The severity of CHV1 effect on methylation levels of infected C. parasitica isolates depended mostly on individual CHV1 strain and on the combination of host and virus genomes, rather than on the virus subtype alone. Furthermore, fungal growth and glutathione S-transferase, catalase and superoxide dismutase activities have been monitored. These enzymes are involved in oxidative stress response and in most cases we observed increase in oxidative stress enzymes’ activity in hypovirulent mycelia coupled with reduced growth of the affected mycelia. However, no clear correlation between the severity of the infection symptoms and virus subtype was determined. The severity of symptoms and the effect on the fungus does not depend on the virus subtype, but rather on the combination of the particular CHV1 and C. parasitica genotype.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Šumarstvo

POVEZANOST RADA