Naslov
A method for measuring area and surface-related parameters on microphotographs by using free and open-source image processing software

Autori
Bijelić, Nikola ; Stolnik, Dunja ; Rođak, Edi ; Lovrić, Ivana ; Belovari, Tatjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th Multinational Congress on Microscopy: BOOK OF ABSTRACTS / Andreja Gajović, Igor Weber, Goran Kovačević, Vida Čadež, Suzana Šegota, Ana Vidoš - Zagreb, 2017, 380-381

ISBN
978-953-7941-19-2

Skup
13th Multinational Congress on Microscopy

Mjesto i datum
Rovinj, Hrvatska, 24.09.2017. - 29.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
histomorphometry ; microscopy ; software ; methods

Sažetak
There are different ways of obtaining volume and surface-related parameters for describing the micromorphology of different tissues, including cancellous bone. Histomorphometry and micro computed tomography can be too costly for small laboratories and institutions, especially when proprietary software or expensive equipment is needed. Free and open-source software alternatives are available today. Some of these can be used for histomorphometry. Here we demonstrate how all image processing and morphometry can be done on freely available software. This method was inspired by the paper by Egan et al. (1), however, we further replaced all of the proprietary and expensive software by using free and open-source alternatives. For measurements of the cancellous bone, images were first processed using open-source and free imaging software GIMP (GNU Image Manipulation Program) and measurements were performed by using FIJI software (FIJI is Just ImageJ), a distribution of ImageJ open-source image processing software (2, 3). The measured parameters were: bone area (B.Ar), trabecular tissue total area (T.Ar) and trabecular bone perimeter (B.Pm). Using several mathematical formulas, we calculated the following parameters describing cancellous bone histomorphometry: trabecular bone volume (BV/TV, in %), trabecular bone surface (BS/TV, in /mm), trabecular thickness (Tb.Th, in mm), trabecular number (Tb.N, in/mm) and trabecular separation (Tb.Sp, in mm). These parameters are important in studies of bone development and quality. There are many benefits of using freely available and open-source software for scientific research, especially the low cost, worldwide availability and transparency, and this work supports that notion.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA