Naslov
New N-9-sulfonylpurine derivatives induce changes in mitochondrial function and ROS accumulation in carcinoma and leukemia cells in vitro

Autori
Jukić, Marijana ; Opačak-Bernardi, Teuta ; Matić, Josipa ; Žinić ; Biserka and Glavaš-Obrovac ; Ljubica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Reactive Oxygen Species and Lipid Peroxidation in Human Health and Disease / - , 2017

Skup
Meeting of the International HNE-Club and the University of Graz, 2017

Mjesto i datum
Graz, Austrija, 14.09.2017. - 15.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
carcinoma cells, leukemia cells, ROS, mitochondrial membrane potential

Sažetak
Our recent studies showed significant antiproliferative capa city of newly synthesized N-9-sulfonylpyrimidine derivatives on human carcinoma, lymphoma, and leukemia cells. These derivatives behave as antimetabolites exploiting cellular metabolism to induce cytotoxicity in treated tumors’ cells (1 -3). Based on prev iously obtained results on N-9-sulfonylpyrimidine derivatives, a new series of N-9-sulfonylpurine derivatives were synthesized and tested on biological potential. The aim of this study was to determine if measured antitumor activity of N-9-sulfonylpurine derivatives is linked with ROS accumulation and mitochondrial membrane destabilization in human cervix adenocarcinoma (HeLa), human chronic myelogenous leukemia (K562) and human Burkitt’s lymphoma (Raji) cells. Changes in the mitochondrial membrane potential (∆Ψm) of tested compounds were determined by flow cytometry, measuring fluorescence of JC-1 dye in tumor cells after 24 hours of treatment. Intracellular accumulation of ROS was determined after 1 hour in cells exposed to N-9-sulfonylpurine derivatives by flow cytometry as well. Tested derivatives induced increased accumulation of ROS and mitochondrial disruption in more than 80% of HeLa and Raji cells. The effect of tested derivatives on K562 cells is reflected by the change of mitochondrial potential in more than 70% with slightly reduced accumulation of ROS. Based on obtained results we can conclude that newly synthesized N-9-sulfonylpurine derivatives are good candidates for further antitumor studies.

Izvorni jezik
Engleski

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