Naslov
Uncovering marine bacterial diversity in the southern Adriatic Sea: from surface to seabed

Autori
Babić, Ivana ; Mejdandžić, Maja ; Sviličić Petrić, Ines ; Bosak, Sunčica ; Mihanović, Hrvoje ; Vilibić, Ivica ; Dupčić Radić, Iris ; Cetinić, Ivona ; Ljubešić, Zrinka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Symposium on Aquatic Microbial Ecology Abstract Book / SAME15 / Gligora Udovič, Marija ; Orlić, Sandi ; Šolić, Mladen - Zagreb : Institut Ruđer Bošković, 2017, 155-155

ISBN
978-953-7941-18-5

Skup
The 15th Symposium on Aquatic Microbial Ecology

Mjesto i datum
Zagreb, Hrvatska, 03.09.2017. - 08.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
marine picoplakton ; next-generation sequencing ; qPCR ; southern Adriatic Sea

Sažetak
High-throughput sequencing was used to determine bacterial diversity, and qPCR for quantify the most represented bacterial groups in the southern Adriatic Sea. The samples were collected from surface to seabed, on total of 16 different depths at four stations in the southern Adriatic Sea, during the late winter BIOTA cruise (Bio-tracing Adriatic Water Masses) conducted in March 2016. The investigated area showed an unusual general circulation that was characterized by mixed layer down up to 200 m, which differed from the usual winter convection event, typical for middle-altitude ecosystems and important for seasonal picoplankton dynamics in the South Adriatic Sea. The bacterial community was dominated with Alphaproteobacteria accounted for the largest fraction (42.31 % of the total) - mainly represented by the SAR11 clade (90.84 %) and Marinimicrobia (18.44 % of the total) represented with the clade SAR406. The bacterial community differed between euphotic and aphotic samples and the highest dissimilarity contribution had OTUs from class Deltaproteobacteria, Bacteroidetes, and Cyanobacteria. Highest abundances of targeted bacterial populations were recorded for Alphaproteobacteria, followed by Gammaproteobacteria and Bacteriodetes. Abundances were found to vary between different sampling points and sampling depths, with values ranging from 8.7 x 103 to 9.13 x 105 genes/mL for Alphaproteobacteria, from 1.44 x 104 to 5.11 x 105 genes/mL for Gammaproteobacteria and from 1.8 x 103 to 2.4x105 genes/mL for Bacteriodetes. For all targeted taxa higher abundances were found in euphotic when compared to aphotic zone.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija

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