Naslov
Acute exposure to hyperbaric oxygenation impaires endothelial nitric oxide production in Sprague-Dawley healthy male rats

Autori
Jukić, Ivana ; Mihaljević, Zrinka ; Rašić, Lidija ; Ćosić, Anita ; Stupin, Ana ; Ezgeta, Ivana ; Drenjančević, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Acta Physiologica 221, S713, 1-290 / - , 2017, 126 (B02-9)

Skup
FEPS 2017

Mjesto i datum
Beč, Austrija, 13.09.2017. - 15.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hyperbaric oxygenation, nitric oxide, endothelium, Sprague-Dawley rats

Sažetak
Question: Previously, study from our laboratory showed that acute exposure to hyperbaric oxygenation (HBO) increase oxidative stress production. This increased oxidative stress caused impaired endothelium-dependent vasodilation while superoxide scavenger TEMPOL in vitro restored that NO-dependent dilation. Present study aimed to determine the effects of acute HBO exposure on flow-induced endothelial NO production. Methods: Healthy male 11 weeks old Sprague-Dawley rats were divided in: CTRL (control group, N=5) and A-HBO (acute single time exposure to HBO, N=5). Exposure to HBO was in hyperbaric oxygen chamber (100% oxygen ; 2 bar/2 hours). Prior to decapitation rats were anesthetized with ketamin and midazolam. Middle cerebral arteries were isolated and cannulated and pressurized at ∆80 mmHg with flow on, on pressure myograph on, in the absence/presence of TEMPOL in vitro. NO production was determined by DAF-2DA to DAF-2T conversion fluorescence assay. All experimental procedures conformed to the European Guidelines for the Care and Use of Laboratory Animals (directive 86/609) and were approved by institutional Ethical Committee. Results: Flow-induced NO production was significantly lower in A-HBO group compared to flow-induced NO production in CTRL group (p=0.020). TEMPOL in vitro increased endothelial NO production in A-HBO group, compared to A-HBO in the absence of TEMPOL (p=0.011). NO production in control group was similar with/without TEMPOL in vitro (p=0.62) suggesting low level of oxidative stress in control rats. Conclusion: Acute exposure to HBO increases oxidative stress. Superoxide scavenging by TEMPOL restores flow-induced NO production in A-HBO group. Funding: research grant VIF2016-MEFOS-19

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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