Naslov
Optimisation of headspace solid phase microextraction for the analysis of polychlorinated biphenyls and organochlorine pesticides in human milk samples

Autori
Brčić Karačonji, Irena ; Jurič, Andreja ; Brajenović, Nataša ; Klinčić, Darija ; Herceg Romanić, Snježana ; Matek Sarić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
53rd Congress of the European Societies of Toxicology (EUROTOX 2017) / Iscan, Mumtaz ; Kandarova, Helena - : Elsevier, 2017, S293-S293

Skup
53rd Congress of the European Societies of Toxicology (EUROTOX 2017)

Mjesto i datum
Bratislava, Slovačka, 10.09.2017. - 13.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
PCB ; OCP ; milk ; SPME ; GC-MS/MS

Sažetak
Polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) are persistent environmental pollutants that raise concern due to their endocrine disrupting, immunotoxic, and neurotoxic properties. Owing to their lipophilic character, they are easily bioaccumulated. Breast milk is a significant source of exposure to these chemicals. A simple and rapid headspace solid phase microextraction (HS-SPME) technique followed by gas chromatography-tandem mass spectrometry (GC- MS/MS) was developed for the simultaneous determination of 21 PCBs and 7 OCPs in human milk samples. The parameters affecting extraction efficiency were simultaneously optimised by experimental design. The best results were obtained using polydimethylsiloxane/divinylbenzene fibre, 0.5 g NaCl added to 1 mL of sample, extraction at 80 °C (40 min), stirring at 300 rpm, and desorption at 260 °C (10 min). The method showed a linear response within the tested concentration range (0.5-20 μg/L), good precision (RSD<10%), accuracy (average 95%), and sensitivity (limit of detection from 0.2 to 2.5 μg/L). The method was applied for the analysis of 30 human milk samples. The most abundant PCBs were 138, 153, 170 and 180. Among the OCPs, p, p´-DDE was the prevalent compound. An overall evaluation of the analytical parameters showed that HS-SPME combined with acquisition in MS/MS mode provides satisfactory sensitivity and selectivity that could replace the time consuming conventional clean-up processes based on liquid–liquid or solid-phase extraction. This work was financially supported by Project No. 8366 funded by the Croatian Science Foundation.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA