Naslov
SOLUBLE FMS-LIKE TYROSINE KINASE-1 (SFLT-1) TO PLACENTAL GROWTH FACTOR (PIGF) RATIO AS AID IN DIAGNOSIS AND PREDICTION OF PREECLAMPSIA – OUR EXPERIENCE

Autori
Horvat, Vesna ; Muller, Andrijana ; Vulin, Martina ; Mandić, Sanja ; Šerić, Vatroslav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical Chemistry and Laboratory Medicine / - , 2017, S758-S758

Skup
22nd IFCC-EFLM European Congress of Clinical Chemistry and Laboratory Medicine. 25th Meeting of the Balkan Clinical Laboratory Federation 15th National Congress of GSCC-CB

Mjesto i datum
Atena, Grčka, 11.06.2017. - 15.06.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sFlt-1, PlGF, ratio, preeclampsia

Sažetak
BACKGROUND: Preeclampsia (PE) is a heterogeneous, multisystem disorder characterized by hypertension, proteinuria and oedema that develop after 20 weeks of gestation. PE affects 2- 5% of pregnant women and remains one of the leading causes of premature birth worldwide. The aim of this study was a clinical validation of prenatal determination of soluble fms-like tyrosine kinase-1 (sFlt-1) to placental growth factor (PIGF) ratio. METHODS: In the study, we included 35 pregnant women, 12 patients with preeclampsia (PE) and 23 women with normal pregnancy outcomes (CTR). Serum samples were collected according to a standard operating procedure at 24 1/7-28 0/7 weeks of gestation and 28 1/7-32 0/7 weeks of gestation and analysed retrospectively. Levels of antiangiogenic sFLT-1 and proangiogenic PIGF were determined by electro- chemiluminescence immunoassay (Cobas e analyzers, Roche Diagnostics Ltd.Mannheim, Germany) and were used to calculate the sFLT-1/PIGF ratio. Based on the results of the ratio, with cut-off ratio value of 34, PE group was subdivided into PE group with elevated sFLT-1/PIGF ratio > 34 (PE+) and PE sFLT-1/ PIGF ratio < 34 (PE-). RESULTS: Patients in the PE+ group had increased incidence of intrauterine growth restriction (IUGR) (67%), lower gestational age at birth (31 GA (29-36 GA)) and lower birth weight of infants (1095 g (810-2060 g)) compared to PE- (17% ; 39 GA (37-40 GA) ; 3230 g (2400-4420 g)) and CTR group (0% ; 39 GA (36- 41 GA) ; 3350 g (2300-4400 g)). The value of sFLT-1/ PIGF ratio was significantly elevated in PE+ group (46.643 (8.989-155.092) and 123.193 (33.831- 217.457)) at both sample collections (p<0.001 ; p<0.001) compared to PE- (2.330 (0.958-9.544) and 3.375 (0.576-18.103) and CTR group (2.618 (1.087-6.180) and 2.599 (0.808-17.890)). There was no difference of age, body mass index (BMI), smoking habits and coagulopathy parameters between these three groups. CONCLUSION: Our results suggest that the sFLT-1/PIGF ratio has the potential to be implemented in clinical practice and could be used as an indicator for the development and estimation of the severity of PE.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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