Naslov
VERIFICATION OF IMPRECISION FOR TSH, FT4 AND FT3 WITH 3 DIFFERENT METHODS

Autori
Omazić, Jelena ; Vuković, Barbara ; Mandić, Sanja ; Horvat, Vesna ; Šerić, Vatroslav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
15th EFLM Continuous Postgraduate Course in Clinical Chemistry and Laboratory Medicine / - , 2015, EA236-eA237

Skup
15th EFLM Continuous Postgraduate Course in Clinical Chemistry and Laboratory Medicine How to assess the quality of your method?

Mjesto i datum
Zagreb, Hrvatska, 24.10.2015. - 25.10.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
imprecision, thyroid-stimulating-hormone, thyroxin, triiodothyronine

Sažetak
Background: In laboratory routine, it is important to ensure that results of analysis are correct and precise. Verification of method is the way for users to check precision and accuracy of results, according to current standards. The aim of this study was to evaluate results for imprecision, obtained from 3 different analyzers, for thyroid- stimulating-hormone (TSH), free thyroxin (FT4) and free triiodothyronine (FT3), regarding to manufacturer’s specifications and desirable specifications for imprecision derived from biological variation. Materials and Methods: The verification of methods was performed on control samples BioRad Lypocheck Immunoassay Plus Control (Biorad Laboratories) (Level1 and Level3) for all 3 thyroid hormones, on Unicel Dxl600 (Beckman Coulter), Architect i1000 (Abbott) and Unauthenticated Download Date | 7/20/17 10:52 AM 15th EFLM Continuous Postgraduate Course in Clinical Chemistry and Laboratory Medicine, 24–25 Oct 2015 eA237 Dimension EXL (Siemens) analyzers. Control samples were tested 10 times in the series (repeatability) and every day in duplicate during 10 days (intermediate precision). Results are shown as coefficient of variation (CV%) and estimated through manufacturer’s and biological variation’s desirable CV% for total imprecision. Statistical analysis was performed using Microsoft Office Excel 2007 software. Results: For all 3 analyzers, CV% for all parameters was within manufacturer’s specifications. CV% (TSH) on all analyzers was within biological variation’s desirable specifications (I = 9.7%). The highest CV was obtained from Architect (I = 6.36%). For FT4 (I = 2.9%) and FT3 (I = 4.0%) the measured values did not fulfilled biological variation’s desirable specifications for neither analyzers. For both, FT4 and FT3, the highest CV was obtained from Unicel Dxl600 (I = 8.36%, I = 8.25%, respectively). Conclusion: All 3 methods have satisfactory imprecision for all 3 hormones, according to manufacturer’s specifications. Biological variation’s specifications are too narrow and they can be accomplished only for TSH. Therefore, each analyzer can be used for routine analyzes of thyroid hormones separately. Future goal is to evaluate accuracy to determine referent analyzer and to compare all 3 methods for these parameters to enable backup for current method.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA