Naslov
Rapid Assessment of Genotoxicity by Flow Cytometric Detection of Cell cycle Alterations

Autori
Bihari, Nevenka

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Fast detection of DNA Damage, Methods and Protocols

Urednik/ci
Didenko, Vladimir

Izdavač
Humana Press

Grad
New York (NY)

Godina
2017

Raspon stranica
13-21

ISBN
978-1-4939-7185-5

Ključne riječi
genotoxicity assessment ; cell cycle alterations ; DNA content ; flow cytometry ; marine mussel ; Mytilus galloprovincialis

Sažetak
Flow cytometry is a convenient method for the determination of genotoxic effects of environmental pollution and can reveal genotoxic compounds in unknown environmental mixtures. It is especially suitable for the analyses of large numbers of samples during monitoring programs. The speed of detection is one of the advantages of this technique which permits the acquisition of 104 to 105 cells per sample in 5 minutes. This method can rapidly detect cell cycle alterations resulting from DNA damage. The outcome of such analysis is a diagram of DNA content across the cell cycle which indicates cell proliferation, G2 arrests, G1 delays, apoptosis and ploidy. Here we present the flow cytometric procedure for rapid assessment of genotoxicity via detection of cell cycle alterations. The described protocol simplifies the analysis of genotoxic effects in marine environments and is suitable for monitoring purposes. It uses marine mussel cells in the analysis and can be adapted to investigations on a broad range of marine invertebrates.

Izvorni jezik
Engleski

Znanstvena područja
Geologija

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