Naslov
Evaluation of in vitro synergy between Tanreqing injection and antibiotics against methicillin- resistant Staphylococcus aureus

Autori
Yang, WeiFeng ; Liu, JueLing ; Blažeković, Biljana ; Sun, YaNan ; Ma, ShuHua ; Ren, ChuanYun ; Vladimir- Knežević, Sanda ; Wang, Yi

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Reviews on Clinical Pharmacology and Drug Therapy / Shabanov, Petr D. - Sankt Peterburg : ECO-vector, LLC, 2017, 13-14

Skup
The 21th International Congress "Phytopharm 2017" and 10th Anniversary of the TCM Research Center Graz

Mjesto i datum
Graz, Austrija, 02.07.2017. - 05.07.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Tanreqing injection ; TCM formula ; synergy ; antibiotics ; methicillin-resistant Staphylococcus aureus
(Tanreqing injection ; TCM formula ; synergy ; antibiotics ; methicillin-resistant Staphylococcus aureus ;)

Sažetak
In light of the emergence and spread of antibiotic resistance and lack of new drugs, herbal drug and antibiotic combination may represent an interesting therapy option for treat multidrug resistant bacteria. The Tanreqing injection is Chinese herbal preparation made from Scutellariae radix (Huang Qin), Ursi fel (Xiong Dan), Naemorhedi cornu (Shan Yang Jiao), Lonicerae flos (Jin Yin Hua) and Forsythiae fructus (Lian Qiao). According to TCM, this formula clears heat and removes phlegm and it is used in China to treat respiratory tract infections (1). The present study was focused on the in vitro antibacterial activity and possible synergistic interactions of Tanreqing injection and selected antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentrations of Tanreqing injection, vancomycin and linezolid were determined by the broth microdilution method (16.5, 0.005 and 0.005 mg/mL, respectively) and synergy was studied by chequerboard technique and time kill curve assay. Obtained values of fractional inhibitory concentration index suggested no interactive effects of the tested combinations of Tanreqing injection and antibiotics. However, the time-kill experiments which are more informative giving an insight into both the rate and extend of antibacterial activity showed that the antibacterial efficacy of vancomycin and linezolid against MRSA was increased through their combination with Tanreqing injection. Combined application of 1/8 MIC vancomycin with 1/4 MIC Tanreqing or 1/8 MIC linezolid with 1/4 MIC Tanreqing revealed the ΔLC24 value of 2.63, suggesting synergistic effects of the tested TCM formula and antibiotics.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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