Naslov
REACTION ENGINEERING OF THE MenD-CATALYZED 1, 4- ADDITION OF α-KETOGLUTARIC ACID TO ACRYLONITRILE

Autori
Sudar, Martina ; Vasić-Rački, Đurđa ; Müller, Michael ; Findrik Blažević, Zvjezdana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biotrans 2017, 13th International Symposium on Biocatalysis and Biotransformations / - : Hungarian Chemical Society, 2017, P-019

ISBN
978-963-9970-76-2

Skup
Biotrans 2017, 13th International Symposium on Biocatalysis and Biotransformations

Mjesto i datum
Budimpešta, Mađarska, 09.07.2017. - 13.07.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mathematical model, MenD, acrylonitrile

Sažetak
Reaction engineering plays an important role in the development of biocatalytic processes, while kinetic modeling combined with reactor modeling provides the basis for the choice of reactor mode and design. Modeling increases our knowledge of the process and leads to a better understanding of the effect of different variables on its outcome. 1, 4-Addition of α- ketoglutaric acid to acrylonitrile catalyzed by thiamine diphosphate (ThDP)-dependent enzyme, 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase (MenD, EC 2.2.1.9.), was studied. This transformation was introduced in 2014. The reaction kinetics and scope of such enzymatic Stetter-type reactions have remained unexplored ; nevertheless, it is known that the products are valuable building blocks. The kinetics of the reaction have been investigated and a mathematical model has been developed. The investigated system is highly complex due to enzyme operational stability decay, acrylonitrile polymerization, and enzyme inhibition. Mathematical modeling proved to be an excellent engineering technique to explain different phenomena that occurred during the investigation of this reaction system. Different reactor set-ups were considered in efforts to avoid the problems of enzyme operational stability decay and enzyme inhibition. It was shown that the reaction product, 6-cyano-4-oxohexanoic acid, can easily be isolated from the reaction mixture.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

Napomena
Project CARBAZYMES-This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 635595.

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