Naslov
Unlocking Cancer Glycomes from Histopathological Formalin-fixed and Paraffin-embedded (FFPE) Tissue Microdissections

Autori
Hinneburg, Hannes ; Korać, Petra ; Schirmeister, Falko ; Gasparov, Slavko ; Seeberger, Peter H. ; Zoldoš, Vlatka ; Kolarich, Daniel

Izvornik
Molecular & cellular proteomics (1535-9476) 16 (2017), 4; 524-536

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cancer Glycome ; FFPE Tissue Microdissections

Sažetak
N- and O-glycans are attractive clinical biomarkers as glycosylation changes in response to diseases. The limited availability of defined clinical specimens impedes glyco- biomarker identification and validation in large patient cohorts. Formalin-fixed paraffin- embedded (FFPE) clinical specimens are the common form of sample preservation in clinical pathology, but qualitative and quantitative N- and O-glycomics of such samples has not been feasible to date. Here, we report a highly sensitive and glycan isomer selective method for simultaneous N- and O-glycomics from histopathological slides. As few as 2000 cells isolated from FFPE tissue sections by laser capture microdissection were sufficient for in- depth histopathology-glycomics using porous graphitized carbon nanoLC ESI-MS/MS. N- and O- glycan profiles were similar between unstained and hematoxylin and eosin stained FFPE samples but differed slightly compared with fresh tissue. This method provides the key to unlock glyco-biomarker information from FFPE histopathological tissues archived in pathology laboratories worldwide.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA