Naslov
Protein and Peptide Separations

Autori
Andjelkovic, Uroš ; Giacometti, Jasminka ; Josic, Djuro

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, pregledni

Knjiga
Liquid Chromatography: Applications Handbooks in Separation Science, 2nd Edition

Urednik/ci
Fanali, Salvatore ; Haddad, Paul R. ; Riekkola, Marja-Liisa ; Poole, Colin

Izdavač
Elsevier

Grad
Amsterdam

Godina
2017

Raspon stranica
107-157

ISBN
978-0-12-805392-8

Ključne riječi
Affinity chromatography ; Chromatography on monoliths ; Displacement chromatography ; Hydrophobic interaction ; Ion exchange ; Size exclusion.

Sažetak
Since the introduction of suitable, highly porous supports and later nonporous and monolithic supports, chromatography has been an indispensible method for protein separations on the analytical and preparative scales. The most frequently used methods for protein chromatography are ion exchange, reversed phase, hydrophobic interaction chromatography, chromatography on hydroxyapatite and different types of affinity, and pseudo-affinity chromatography. Because of the use of organic solvents during the separation in reversed-phase chromatography, denaturation and loss of biological activity frequently occurs, and this method is less suitable for separation of biologically active, therapeutic proteins. Chromatography on monolithic supports and chromatography in the displacement mode offer additional opportunities for fast, highly effective separation of proteins on both the analytical and preparative scales.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA