Naslov
The antimicrobial effect of photodynamic therapy on the surfaces of dental implants – in vitro pilot study

Autori
Azizi ; Bleron ; Budimir, Ana ; Mehmeti, Blerim ; Gabrić, Dragana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
37th ASLMS Annual Conference. U: Lasers in Surgery and Medicine 2017 ; 49(4) / Wong, Brian - New York (NY) : John Wiley & Sons, 2017, 435-436

Skup
37th ASLMS Annual Conference

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 05.04.2017. - 10.04.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
dental implants ; surface ; photodynamic therapy ; contamination

Sažetak
Background: When treating peri implantitis, the decontamination of the implant surfaces is one of the main goals. The use of photodynamic therapy, has emerged as a new and potentially efficient decontamination method. The aim of this study was to determine the antimicrobial effect of photodynamic therapy using two different light sources on dental implants contaminated with anaerobic bacteria. Study: For this pilot study, 12 titanium dental implants were used. The implants were put into bacterial suspension prepared from three different bacteria: Prevotella intermedia, Aggreggatibacter actinomycetemcomitans and Porphyromonas gingivalis. for 72 hours in anaerobic conditions. After contamination, the implants were divided into three groups (two treatment groups and one control group). The implants belonging to the first treatment group were coated with a toluidine based photosensitive dye for 60 seconds, and then treated for 60 seconds with a light source with 660 nm wavelength, and 320mm fiber tip (Hager & Werken). The implants belonging to the second treatment group were coated with methylene blue based photosensitive dye for 60 seconds, and then treated for 60 seconds with a light source with 660 nm wavelength, and 3D Pocket Probe (Bredent Medical). The negative control group did not receive any treatment. After treatment, the implants were placed into test tubes containing 2 ml phosphate buffered saline, they were vortexed for 60 seconds and 200 ul from each tube was spread into Columbia agar plates for 72 hours. Colony Forming Units (CFU) were counted and the data were compared to the control group. Results: The results show that there is statistically significant bacterial reduction in both treatment groups: >99% bacterial reduction ; p < 0.05, however there is no statistically significant difference between the two treatment groups (p > 0.05). Conclusion: Based on our study, antimicrobial photodynamic therapy can achieve significant decontamination of the implant surfaces, however the total resolution of the bacteria is not achieved.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

Napomena
Erratum Re: “2017 ELECTRONIC POSTERS (ePosters), ”
Lasers in Surgery and Medicine, 2017 ; 49(4):417–
467 When originally published, the abstract for
ePoster E50 (The Antimicrobial Effect of
Photodynamic Therapy on the Surfaces of Dental
Implants – In Vitro Pilot Study) was missing one
of the author’s names. Dragana Gabric is a
coauthor. She is affiliated with the University of
Zagreb, School of Dental Medicine Zagreb, Zagreb,
Croatia.

POVEZANOST RADA