Pregled bibliografske jedinice broj: 883192
WHO TOUCHED THAT? APPLYING CLASSIC STR MULTIPLEX SYSTEMS TO TOUCH DNA
WHO TOUCHED THAT? APPLYING CLASSIC STR MULTIPLEX SYSTEMS TO TOUCH DNA // 8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine, Split, Croatia, Abstract book
Split, Hrvatska, 2013. (poster, nije recenziran, sažetak, ostalo)
CROSBI ID: 883192 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
WHO TOUCHED THAT? APPLYING CLASSIC STR MULTIPLEX SYSTEMS TO TOUCH DNA
Autori
Zoko, M. ; Bilić, S. ; Car, I. ; Marjanović, Damir
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo
Izvornik
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine, Split, Croatia, Abstract book
/ - , 2013
Skup
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine
Mjesto i datum
Split, Hrvatska, 24.06.2013. - 28.06.2013
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
Touch DNA, STR MULTIPLEX SYSTEMS,
Sažetak
The aim of this study was to test the possibilities of touch DNA processing using standard STR multiplex kits: PowerPlex® 16 and AmpFLSTR® Identifiler® Plus. For the purpose of the study, a total of 18 swab specimens were processed: 8 door knobs, 5 office chair armrests, 3 light switches, 1 laptop key and 1 mouse button. In order to obtain maximum DNA yield, isolation was conducted according to Qiagen protocols, Investigator Kit® for the PowerPlex® samples and Micro Kit® for the Identifiler® samples. Additionally, samples processed using PowerPlex® kit were put through speed-vac and 32 PCR cycles. Likewise, the Identifiler® samples were further purified and concentrated using Microcon® filters. We observed par- tial mixture profiles, as well as allele dropout, suggesting the amount of DNA was within the area of low copy number DNA. A single sample, one of the armrests, contributed a full DNA profile. As for the mixed DNA profiles that were also detected, it was very hard to ascertain whether alleles were not in fact allele drop- ins or misinterpreted stutters. Most of the profiles were unsuitable for statistical analysis and were inconclusive when compared to reference samples. Nevertheless, some were fairly informative and could prove useful as part of a more complex investigation. The completely generated DNA profile was success- fully connected to a donor profile
Izvorni jezik
Engleski
