Pregled bibliografske jedinice broj: 883178
Polymorphism of 15 STR Loci in the Turkish Population of Bosnia and Herzegovina
Polymorphism of 15 STR Loci in the Turkish Population of Bosnia and Herzegovina // 8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine Abstract book
Split, Hrvatska, 2013. (poster, nije recenziran, sažetak, ostalo)
CROSBI ID: 883178 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Polymorphism of 15 STR Loci in the Turkish Population of Bosnia and Herzegovina
Autori
Dogan S. ; Kovačević, L. ; Čakar, J. ; Džehverović, M. ; Hadžić, N. ; Pilav, A. ; Memon, AR. ; Marjanović, Damir
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo
Izvornik
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine Abstract book
/ - , 2013
Skup
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine
Mjesto i datum
Split, Hrvatska, 24.06.2013. - 28.06.2013
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
STR loci, Polymorphism, Turkish Population
Sažetak
Allele frequencies of 15 STRs included in the PowerPlex 16 System (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, VWA, D8S1179, TPOX and FGA) were calculated from the referent sam- ple of 100 unrelated individuals of both sexes from the Turkish population living in Bosnia and Herzegovina. Buccal swabs, as a source of DNA, were collected from volunteers who gave the informed consent. DNA extraction was performed using QIAamp DNA Micro kit. Two ng of DNA template was used to amplify 15 STR loci by PCR multiplex amplification which was performed by using the PowerPlex 16 kit (Promega Corp., Madison, WI, USA) according to the manufacturer’s protocol. The amplifications were carried out in a PE Gene Amp PCR System thermal cycler (Applied Biosystems) and capillary electrophoresis was carried out in an ABI PRISM 310 Genetic Analyzer (Applied Biosystems) in accordance with the manufacturer’s recommendations. The frequency of each locus was calculated from the numbers of each observed genotype. Deviation from Hardy- Weinberg equilibrium and observed heterozygosity were calculated. Data were analyzed by using Microsoft Excel workbook template - Powerstats V12 and the power of discrimination (PD), power of exclusion (PE), as well as other population genetic indices for the 15 STR loci were calculated. Obtained results contribute to existing Turkish DNA database, as well as insight of differences and similarities in comparison to population of Bosnia and Herzegovina.
Izvorni jezik
Engleski
