Naslov
Kinship Analysis of Ancient Individuals by Combined Use of Small Biallelic Insertion/Deletion (INDEL) and Short Tandem Repeats (STR).

Autori
Topčagić, J. ; Kovačević, L. ; Čakar, J. ; Hadžić, N. ; Džehverović, M. ; Pilav, A. ; Marjanović, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine, Split, Croatia, Abstract book / - , 2013

Skup
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine

Mjesto i datum
Split, Hrvatska, 24.06.2013. - 28.06.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
bioarchaeology , kinship, INDEL, STR

Sažetak
In bioarchaeological research, the results of DNA typing are often used for sex determination and kinship analysis among ancient individuals. However, the amount of information and the reliability of the results in such analyses are limit- ed by the quality and quantity of isolated ancient DNA (aDNA). The aim of this study was to investigate the possibility of determining putative familial relation- ships between three ancient individuals using commercially available short tandem repat (STR)-multiplex and insertion/deletion (INDEL)-multiplex kits suitable for degraded DNA samples and those containing PCR inhibitors. Three tooth samples were obtained from human skeletal remains of Vranjevo selo, an archaeological site located in Bosnia and Herzegovina. DNA was extracted using optimized phe- nol-chloroform protocol with EDTA decalcification pre-extraction step. DNA sam- ples were then quantified using the Quantifiler™ Human DNA Quantification Kit. The Investigator® DIPplex Kit was used for multiplex amplification of 30 small bial- lelic INDELs, and STR analysis was performed using the Powerplex® ESX17 and ESI17 kits. Both types of kits also include the Amelogenin locus. DNA fragment size analyses were conducted on an ABI PRISM 310 Genetic analyzer. At each stage, requirements for preventing contamination with exogenous DNA were achieved. Kinship analyses were carried out using Familias program. Complete or partial DNA profiles were obtained from all three samples. The results of kinship analyses demonstrated the presence of genetic relationship between three ancient individuals, in accordance with anthropological and archaeological data. The over- all results indicate the suitability of improved commercially available multiplex kits for the analysis of aDNA.

Izvorni jezik
Engleski

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