Naslov
Polymorphism of Six New Y Chromosome STR Loci (DYS576, DYS481, DYS549, DYS533, DYS570, DYS643) in Referent Sample of Bosnia and Herzegovina

Autori
Fatur Cerić, V. ; Kovačević, L. ; Hadžić, N. ; Čakar J. ; Primorac, D. ; Marjanović, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine, Split, Croatia, Abstract book / - , 2013

Skup
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine

Mjesto i datum
Split, Hrvatska, 24.06.2013. - 28.06.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
STR markers, Polymorphism, Bosnia and Herzegovina

Sažetak
STR markers are useful in establishing paternal linkages, kinship testing and familial searching, also finds its application in studying migration pattern of populations. Y STR haplotyping is particularly important for forensic analysis in case of rape, especially in those cases where more than one male was involved. New kit PowerPlex® Y23 enables the coamplification and 5- dye detection of 23 loci, 6 new loci(DYS576, DYS481, DYS549, DYS533, DYS570, DYS643) in comparison to Applied Biosystems and 11 new loci in comparison to previous PowerPlex Y kit which included 12 STR loci. Aim of this study was to indicate the significance of increasing the number of STR loci in forensic analysis. This study was conducted on referent sample of 100 unrelated males from Bosnia and Herzegovina. Genomic DNA was extracted from buccal swab using salting out method as well as QiagenDNeasy™ Tissue Kit. PCR amplifications have been carried out in PE GeneAmp PCR System Thermal Cycler. Y Chromosomal 23 STRs alleles were typed using ABI 310 Genetic Analyzer. Estimation of haplotype frequencies, haplotype diversity and average gene diversity were done using Arlequin Software V. 3.5. Ninety-eight unique haplotypes were detected, and one appeared two times. The most polymorphic locus is DYS418 on which we detected 14 alleles. Furthermore on this DYS418 locus we detected allele 33 which was not incorporated in allelic ladder provided by PowerPlex® Y23 kit. The least polymorphic loci in our study were DYS389I, DYS391, DYS437 and DYS393.

Izvorni jezik
Engleski

POVEZANOST RADA