Pregled bibliografske jedinice broj: 883134
Direct PCR Optimization
Direct PCR Optimization // 8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine, Split, Croatia, Abstract book
Split, Hrvatska, 2013. (poster, nije recenziran, sažetak, ostalo)
CROSBI ID: 883134 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Direct PCR Optimization
Autori
Hadžić, N. ; Čakar, J. ; Kovačević, L. ; Džehverović, M. ; Pilav, A. ; Marjanović, Damir
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo
Izvornik
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine, Split, Croatia, Abstract book
/ - , 2013
Skup
8th ISABS Conference in Forensic, Anthropologic and Medical genetics and Mayo Clinic Lectures in Translational Medicine
Mjesto i datum
Split, Hrvatska, 24.06.2013. - 28.06.2013
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
PCR model, The PowerPlex® 18D System, 16 STR loci
Sažetak
Lately, with the aim of easier, faster and cheaper analysis of the referent samples, collected for various purposes and primarily for the creating forensic DNA data- bases as well as rapid paternity testing, the procedures that completely bypass the step of DNA extraction from this type of specimens are optimized. This contributes significantly to saving time needed for samples processing, which is ultimately the primary goal. Namely, the direct PCR model entails the amplification from the buccal mucosa swabs and blood samples collected by some of the different types of collectors. In Total, 28 buccal swab samples were processed. The PowerPlex® 18D System was applied, which allows the direct amplification of 18 STR loci, 13 CODIS loci, and Penta E, Penta D, D2S1338, D19S433, as well as of amelogenin, and certainly enhances the power of individual discrimination due to extended range of loci. The total volume of the individual reaction mixture was 25 µL. The amplification of samples was performed using the PCR thermocycler, according to the manufacturer’s recommendations. Detection of the results was performed on an ABI PRISM® 310 genetic analyzer. Given that the two Promega kits share 16 STR loci, it was possible to examine the accuracy of the detected alle- les using PowerPlex® 18D by comparing them to the alleles detected using PowerPlex® 16 multiplex of the same persons, thus determining the optimal method of collecting and processing this type of biological traces by the use of direct amplification kit.
Izvorni jezik
Engleski
