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Pregled bibliografske jedinice broj: 881434

Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces

Pigac, Jasenka; Vujaklija, Dušica; Toman, Zora; Gamulin, Vera; Schrempf, Hildgund
Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces // Plasmid, 19 (1988), 3; 222-230 doi:10.1016/0147-619X(88)90040-6 (međunarodna recenzija, članak, znanstveni)

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Naslov
Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces

Autori
Pigac, Jasenka ; Vujaklija, Dušica ; Toman, Zora ; Gamulin, Vera ; Schrempf, Hildgund

Izvornik
Plasmid (0147-619X) 19 (1988), 3; 222-230

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
structural instability ; bifunctional plasmid

Sažetak
Structural instability of a hybrid plasmid pZG1, consisting of Escherichia coli pBR322 and Streptomyces pIJ350 plasmids, has been studied in Streptomyces. After transformation of S. lividans 1326 and S. rimosus R6 protoplasts with pZG1, transformants harbored the intact pZG1 and various deleted plasmid forms. The pattern of deleted plasmids varied with the transformant colony age and changed upon subcultivation. The presence of intact pZG1 in at least a part of the Streptomyces colonies indicated that the plasmid was capable of replicating in the transformants and that deletion events occurred after at least one round of replication. Less instability of pZG1 in S. rimosus R6 was observed when this strain was transformed with the DNA isolated from the same strain. pZG1 and its various derivatives were found in S. lividans 1326 and S. rimosus R6 as double- and single-stranded DNA molecules. Structural instability of pZG1 could therefore be due at least in part to the presence of single- stranded DNA.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb,
Pliva-Istraživački institut,
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Jasenka Pigac (autor)

Avatar Url Vera Gamulin (autor)

Avatar Url Dušica Vujaklija (autor)

Avatar Url Zora Toman (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com doi.org

Citiraj ovu publikaciju:

Pigac, Jasenka; Vujaklija, Dušica; Toman, Zora; Gamulin, Vera; Schrempf, Hildgund
Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces // Plasmid, 19 (1988), 3; 222-230 doi:10.1016/0147-619X(88)90040-6 (međunarodna recenzija, članak, znanstveni)
Pigac, J., Vujaklija, D., Toman, Z., Gamulin, V. & Schrempf, H. (1988) Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces. Plasmid, 19 (3), 222-230 doi:10.1016/0147-619X(88)90040-6.
@article{article, author = {Pigac, Jasenka and Vujaklija, Du\v{s}ica and Toman, Zora and Gamulin, Vera and Schrempf, Hildgund}, year = {1988}, pages = {222-230}, DOI = {10.1016/0147-619X(88)90040-6}, keywords = {structural instability, bifunctional plasmid}, journal = {Plasmid}, doi = {10.1016/0147-619X(88)90040-6}, volume = {19}, number = {3}, issn = {0147-619X}, title = {Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces}, keyword = {structural instability, bifunctional plasmid} }
@article{article, author = {Pigac, Jasenka and Vujaklija, Du\v{s}ica and Toman, Zora and Gamulin, Vera and Schrempf, Hildgund}, year = {1988}, pages = {222-230}, DOI = {10.1016/0147-619X(88)90040-6}, keywords = {structural instability, bifunctional plasmid}, journal = {Plasmid}, doi = {10.1016/0147-619X(88)90040-6}, volume = {19}, number = {3}, issn = {0147-619X}, title = {Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces}, keyword = {structural instability, bifunctional plasmid} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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