Pregled bibliografske jedinice broj: 881428
Identification of an A-factor-dependent promoter in the streptomycin biosynthetic gene cluster of Streptomyces griseus
Identification of an A-factor-dependent promoter in the streptomycin biosynthetic gene cluster of Streptomyces griseus // MGG. Molecular & general genetics, 229 (1991), 1; 119-128 doi:10.1007/BF00264220 (međunarodna recenzija, članak, znanstveni)
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Naslov
Identification of an A-factor-dependent promoter
in the streptomycin biosynthetic gene cluster of
Streptomyces griseus
(Identification of an A-factor-dependent promoter
in the streptomycin biosynthetic gene cluster of
Streptomyces griseus.)
Autori
Vujaklija, Dušica ; Ueda, Kenji ; Hong, SK ; Beppu, Teruhiko ; Horinouchi, Sueharu
Izvornik
MGG. Molecular & general genetics (0026-8925) 229
(1991), 1;
119-128
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
A-factor ; streptomyces-griseus ; streptomycin biosynthesis ; streptomycin resistance ; gene expression
Sažetak
A-factor (2-isocapryloyl-3 R-hydroxymethyl-gamma- butyrolactone) is a microbial hormone controlling streptomycin (Sm) production, Sm resistance and sporulation in Streptomyces griseus. In order to identify A-factor-dependent promoters in the Sm biosynthetic gene cluster, a new promoter-probe plasmid with a low copy number was constructed by using an extremely thermostable malate dehydrogenase gene as the reporter. Of the three promoters in the Sm production region that includes strR, aphD and strB, only the promoter of strR, which codes for a putative regulatory protein, was found to be directly controlled by A- factor. This was also confirmed by S1 nuclease mapping. The region essential for its A-factor- dependence was determined to be located 430-330 base pairs upstream of the transcriptional start point. Increase in the copy number of the strR promoter region did not lead to a corresponding increase in the total promoter activity, probably due to titration of a putative activator which binds to the enhancer-like region and controls the expression of the strR promoter. This putative activator is apparently distinct from the A- factor-receptor protein. The aphD gene, which encodes the major Sm resistance determinant, Sm-6- phosphotransferase, was transcribed mainly by read-through from the A-factor-dependent strR promoter ; this accounts for the prompt induction of Sm resistance by A-factor.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
