Pregled bibliografske jedinice broj: 881407
Direct transfer of plasmid DNA between Streptomyces spp. and Echerichia coli by electroduction
Direct transfer of plasmid DNA between Streptomyces spp. and Echerichia coli by electroduction // Journal of antibiotics, 48 (1995), 7; 635-637 doi:10.7164/antibiotics.48.635 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 881407 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Direct transfer of plasmid DNA between Streptomyces
spp. and Echerichia coli by electroduction
Autori
Vujaklija, Dušica ; Davies, Julian
Izvornik
Journal of antibiotics (0021-8820) 48
(1995), 7;
635-637
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Electroduction ; E.coli ; Streptomyces spp.
Sažetak
We describe a simple and reliable method which allows the direct transfer of shuttle plasmids between Streptomyces spp. and E. coli. The method is based on the fact that plasmid DNA molecules can be released or taken up from cells under conditions of electroporation. When a suspension of a plasmid- containing Streptomyces spp. is mixed with electroporation-competent E. coli and submitted to an electric pulse, plasmid DNA transfer to the E. coli recipient takes place. Two different Streptomyces spp. (S. lividans TK23, or TK24 and S. rimosus 554w) were effective donors, and the method was successfully employed to transfer four different bifunctional vectors (pPM801, pFD666, pRL270X and pZG5) varying in size from 5.2 similar to 14.4kb, to E. coli. This provides a convenient method for the analysis of Streptomycete transformants.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- PubMed
- Index medicus
