Naslov
Satellite DNA conserved between species Palorus ratzeburgii and Tenebrio molitor

Autori
Meštrović, Nevenka ; Durajlija, Sonja ; Ugarković, Đurđica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Godišnji sastanak hrvatskih biokemičara, Supetar, 22.09.-25.09. 1995 / Glojnarić Spasić, Ines - : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 1995, 108-108

ISBN
953-6256-16-9

Skup
Annual meeting of Croatian Biochemists, Supetar, 22.09.-25.09. 1995

Mjesto i datum
Supetar, Hrvatska, 22.09.1995. - 25.09.1995

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
satellite DNA, centromere, Southern blot analysis, fluorescence in situ hybridization, Palorus ratzeburgii, Tenebrious molitor

Sažetak
Satellite DNAs often show variability in sequence even among closely related species. Palorus ratzeburgii and Tenebrio molitor belong to the family Tenebrionidae (Coleoptera, Insecta). Each of these species contains very abundant and homogeneous satellite DNA which constitutes 50% of T. monitor and 40% of P. ratzeburgii genome. Although the satellites have the same monomer length of 142 bp and very similar tertiary structure, they share no homology at the nucleotide sequence level. Southern blot hybridisation revealed existence of the sequences in T. monitor genome that hybridised with cloned P. ratzeburgii main satellite dimer under medium stringency conditions. These sequences represent about 0, 1% of T. molitor genome. P. ratzeburgii satellite like sequences from Sau3A digestion of T. molitor DNA have been cloned and recombinants were tested by colony hybridization. Sequence analysis of positive clones has been performed. Fluorescent in situ hybridisation showed the location of P. ratzeburgii satellite sequences in centromeric portion all T. monitor chromosomes, among blocks of main satellite DNA. Sequences like these, conserved in related species, may be very useful for the study of satellite DNA evolution as well as for phylogenetic studies of closely related taxa.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA