Naslov
Octopus vulgaris immune response at gene expression level by in vitro immunostimulation of hemocytes

Autori
Petrić, Mirela ; Baldascino, Elena ; Lauritano, Chiara ; Zarrella, Ilaria ; Ponte, Giovanna ; Fiorito, Graziano

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the 17th International Conference on Diseases of Fish and Shellfish / Mladineo, Ivona - Las Palmas, 2015, 208-208

Skup
17th EAFP International Conference on Diseases of Fish and Shellfish

Mjesto i datum
Las Palmas de Gran Canaria, Španjolska, 07.09.2015. - 11.09.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Octopus vulgaris, immunostimulation, hemocytes

Sažetak
Investigations of the cephalopod immune system have recently become of great importance due to the increasing interest for aquaculture, for the role of potential pathogens on the quality of the “sea-products” for human consumption and for concern about cephalopod welfare since the inclusion of this taxon in the Directive 2010/63/EU on the protection of animals utilized for scientific purposes. Cephalopods rely solely on innate immunity and hemocytes are suggested to play a key role in the immune-system response to challenges (e.g. through phagocytosis, encapsulation, infiltration of pathogens). Aim of this research was to study the elements of cephalopod immune response at gene expression level by in vitro immunostimulation of Octopus vulgaris hemocytes. Hemolymph cell suspension was plated with Squid Ringer’s Solution and kept at 18 °C prior immune-stimulation with commercially available immunostimulants: LPS (Escherichia coli lipopolysaccharides) as bacterial imitator, Poly I:C (polyinosinic-polycytidylic acid sodium salt) as viral imitator and zymosan, a yeast cell extract eliciting fungal type infection response. As control, hemocytes were kept under the same conditions as the immunostimulated ones. Immunostimulation was accomplished within different time- points: 1, 4, 24 and 48 hours. At the end of each time-point hemolymph suspension was collected and centrifuged following pellet resuspension in TriReagent. Total RNA was extracted from hemocytes following manufacturer’s protocol (InVitrogen) and RNA samples were retrotranscripted into cDNA, which was utilized as template for PCR and RT-qPCR analyses. The expression levels of specific gene of interest, immune-related genes and genes associated to the antioxidant system, stress and detoxification, was analyzed. In general, for all the treatments (LPS, Poly I:C and zymosan) and the time points (1, 4, 24 and 48 h) the majority of investigated antioxidant genes (sod, gpx and gsh-s) were up-regulated after 1 and 4 h post-stimulation, and the majority of the investigated immune genes (nfkb, ser and tnf) appeared up-regulated only after 24 or 48 h post- stimulation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

Napomena
COST Action FA1301 - CephsInAction

POVEZANOST RADA