Naslov
TOTAL PLASMA N-GLYCOME IN ALLERGEN SENSITIZED CHILDREN

Autori
Šimunović, Jelena ; Devereux, Graham ; Zaitseva, Olga ; Vilaj, Marija ; Trbojević- Akmačić, Irena ; Vučković, Frano ; Lauc, Gordan ; Pezer, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
12th Jenner Glycobiology and Medicine Symposium on TRANSLATIONAL GLYCOBIOLOGY, From Bench to Bedside

Mjesto i datum
Dubrovnik, Hrvatska, 06.05.2017. - 09.05.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Total plasma N-glycome, allergy, biomarker, children

Sažetak
Total plasma N-glycome (TPNG) is known to change with respect to a disrupted homeostasis and different diseases involving systemic inflammation, but its content has never been systematically analysed in allergic diseases. The aim of this study was to analyse TPNG in allergen-sensitized children and evaluate its potential as an early biomarker for sensitization state. Total plasma N-glycans from 283 10-year-old children of both sexes (101 sensitized to dog, cat, timothy grass, egg, peanut and/or house dust mite ; 182 non- sensitized) were released by PNGase F following protein denaturation by sodium dodecyl sulfate. The released N glycans were labeled with 2 aminobenzamide and analyzed by hydrophilic interaction ultraperformance liquid chromatography (UPLC). First results indicate there is no difference in TPNG in allergen- sensitized vs. non-sensitized children.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA