Naslov
DRIED BLOOD SPOTS AS SOURCE MATERIAL FOR IMMUNOGLOBULIN G N-GLYCAN ANALYSIS

Autori
Šimunović, Jelena ; Jurić, Julija ; Trbojević- Akmačić, Irena ; Momčilović, Ana ; Nakić, Natali ; Lauc, Gordan ; Pezer, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
12th Jenner Glycobiology and Medicine Symposium on TRANSLATIONAL GLYCOBIOLOGY, From Bench to Bedside

Mjesto i datum
Dubrovnik, Hrvatska, 06.05.2017. - 09.05.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Immunoglobulin G, N-glycans, dried blood spots

Sažetak
IgG glycans are emerging as a new putative biomarker for different diseases and are routinely released from IgG isolated from plasma or serum samples. Since dried blood spots (DBS) have many advantages over plasma or serum samples (reduction of sample volume, simplification of the collection, storage and transport procedures, decreased cost of analysis), the aim of this study was to evaluate DBS as source material for immunoglobulin G N- glycan analysis. IgG was isolated from DBS by affinity chromatography on a protein G monolithic plate and its N glycans released by PNGase F, labeled with 2 aminobenzamide and analyzed by hydrophilic interaction ultraperformance liquid chromatography (UPLC). DBS proved to be a good source material for IgG N-glycan analysis by UPLC. The analytical procedure is very robust if DBS are processed on the day of sample collection. In order to obtain temporally stable quantification of IgG N- glycans by UPLC, DBS storage conditions have to be optimized.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA