Naslov
Different combinations of maternal and postnatal diet are reflected in changes of hepatic parenchyma and hepatic TNFalpha expression in male rat offspring

Autori
Perić Kačarević, Željka ; Grgić, Anđela ; Šnajder, Darija ; Bakula, Marina ; Bijelić, Nikola ; Lekšan, Igor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Obesity Facts / Johannes Hebebrand - Freiburg : Karger Publishers, 2017, 65-65

ISBN
978-3-318-06069-0

Skup
24th European Congress on Obesity (ECO2017)

Mjesto i datum
Porto, Portugal, 17.05.2017. - 20.05.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
obesity, TNF-alpha, rat, liver

Sažetak
Introduction: Obesity is related to increased TNF-alpha production in different tissues and TNF-alpha is connected to mitochondrial dysfunction in the liver and development of fatty infiltration of the liver. Also, postnatal change from normal to high-fat diet causes a significant increase in TNF-alpha serum levels. The aim of this research was to determine how maternal diet during pregnancy and lactation and switching male offspring to a different dietary regime influences rat liver. Methods: Ten female Sprague Dawley rats at nine weeks of age were randomly divided in two groups and fed either standard laboratory chow or high-fat diet during five weeks, and then mated with the same male subject. After birth and lactation male offspring from both groups were further divided into four subgroups depending on their subsequent diet. At 22 weeks of age, the animals were weighted, sacrificed and major organs were collected and weighted. Immunohistochemistry for TNF-alpha was performed on liver, and liver samples were analysed for pathohistological changes. Results: The group in which mothers were fed standard chow and offspring high-fat diet had the most pronounced changes being the heaviest liver, poorest histopathological findings and strongest TNF-alpha immunohistochemical staining of liver parenchyma. Conclusion: High- fat diet during pregnancy and lactation and switching to high-fat diet postnatally affects liver weight, histological structure and TNF- alpha expression.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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