Naslov
Localization of spindle assembly checkpoint proteins in mouse spermatocytes

Autori
Keser, Vanessa

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, preddiplomski

Fakultet
Prehrambeno-biotehnološki fakultet

Mjesto
Zagreb

Datum
20.06

Godina
2016

Stranica
25

Mentor
Štafa, Anamarija

Neposredni voditelj
Kauppi, Lissa

Ključne riječi
spindle assembly checkpoint, bipolar attachment, Spo11β-only mice

Sažetak
During meiosis, appropriate segregation of chromosomes is of crucial importance, and it is achieved via bipolar orientation of kinetochore-microtubule attachment. Spindle assembly checkpoint (SAC), through a specific SAC complex consisting of MAD1, MAD2, BUB1 and 3F3/2 phosphoepitose and several other proteins, delays anaphase onset until all chromosomes are properly attached to the spindle. In case of malfunctioning SAC, cell will enter anaphase without proper kinetochore- microtubule orientation, and improper chromosomes segregation will occur, resulting in abnormal number of chromosomes in daughter cell. In this work proteins MAD2 and 3F3/2 phosphoepitope of the SAC complex were immunostained in order to determine their localization. Due to more frequent improper mitotic kinetochore-microtubule attachment, compared to the wild type, transgenic Spo11β- only mice were used. It was determined that both MAD2 and 3F3/2 phosphoepitope colocalize with SYCP3 in metaphase I.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA