Naslov
Construction of Saccharomyces cerevisiae hybrid diploids for bioethanol production

Autori
Žunar, Bojan ; Pranklin, Andrea ; Lončar, Ana ; Nestić, Davor ; Svetec Miklenić, Marina ; Štafa, Anamarija ; Šantek, Božidar ; Svetec, Ivan- Krešimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EBTNA 2016 / - Riga, 2016

Skup
EBTNA 2016

Mjesto i datum
Riga, Latvija, 05.05.2016. - 07.05.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hybrid diploids, bioethanol production

Sažetak
Bioethanol production from raw lignocelullosic material requires a strain of a microorganism which tolerates high ethanol concentrations and presence of growth inhibitors. Therefore we constructed hybrid diploid S.cerevisiae strains by mating two haploids having desirable traits. One haploid was isolated from wine thus tolerating high ethanol concentration and the other has been shown to be resistant to several growth inhibitors. These haploids were mated and resulting homozygous and heterozygous (hybrid) diploids were tested for the ability to produce ethanol and to grow in the presence of several growth inhibitors frequently found in different lignocelullosic hydrolysates (acetic and levulinic acid, 2-furaldehyde). The results showed that some hybrid diploids were more resistant to tested inhibitors than parental haploid strains and homozygous diploids. It is also interesting that constructed strains showed different fermentation ability in CO2 production test, suggesting genetic variability of constructed hybrids. All together, these results suggest that the approach used in this study may help to construct new strains as well as to improve traits of biotechnologically interesting strains.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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