Naslov
Antisense RNA silencing of the TROL protein involved in plant photosynthetic energy partitioning

Autori
Fulgosi, Hrvoje ; Jurić, Snježana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st COST iPlanta Conference - Creating a plant RNAi Research network COST Action CA15223 : Book of abstracts / Mezzetti, Bruno - Rim : University Sapienza, 2017

Skup
1st COST iPlanta Conference - Creating a plant RNAi Research network COST Action CA15223

Mjesto i datum
Rim, Italija, 15.02.2017. - 17.02.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
feredoksin NADP+ oksidoreduktaza ; fotosustav I ; transformacija pomoću agrobakterija, Arabidopsis thaliana
(ferredoxin NADAP+ oksidoreductase ; photosystem I ; agrobacterium-mediated plant transformation ; Arabidopsis thaliana)

Sažetak
Although basic mechanisms of plant photosynthesis have been established, regulatory pathways of energy partitioning are still largely unknown. We have proposed that At4g01050 gene product (TROL protein) from Arabidopsis thaliana (L.) Heynh., due to its catalytically inactive rhodanese domain and FNR binding, participates in regulation of photosynthetic electron partitioning (1). Many know catalytically inactive rhodanese domains are implicated in regulation, most commonly linked with signalling processes. Here we present reverse genetic approach to study the functions of the At4g01050 gene by using antisense RNA silencing. The At4g01050 cDNA was inserted into pGPTVBAR binary vector in the antisense orientation, with respect to CaMV 35S promoter. Arabidopsis plants were subsequently transformed with pGPTV-antiTROL construct by using agroinfiltration. Plants with silenced TROL were subsequently analysed on molecular, biochemical, and cell biology levels (2). References: 1. L. Vojta, L. D. Carić, V. Cesar, J. Antunović Dunić, H. Lepeduš, M. Kveder, H. Fulgosi: TROL-FNR interaction reveals alternative pathways of electron partitioning in photosynthesis. Scientific Reports 5, 10085 ; doi: 10.1038/srep10085 (2015). 2. S. Jurić, K. Hazler Pilepić, A. Tomašić, H. Lepeduš, B. Jeličić, S. Puthiyaveetil, T. Bionda, L. Vojta, J. F. Allen, E. Schleiff, H. Fulgosi: Tethering of ferredoxin:NADP+ oxidoreductase to thylakoid membranes is mediated by novel chloroplast protein TROL. The Plant Journal. 60 (2009): 783-794.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA