Naslov
High level colistin resistance in Acinetobacter baumannii isolate from patient with mediastinitis after coronary artery bypass graft and aortic valve replacement

Autori
Rubić, Žana ; Marović, Zlatko ; Šeruga Musić, Martina ; Škorić, Dijana ; Novak, Anita ; Tonkić, Marija ; Goić Barišić, Ivana ; Hrenović, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
27th European Congress of Clinical Microbiology and Infectious Diseases

Mjesto i datum
Beč, Austrija, 22.04.2017. - 25.04.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Acinetobacter baumannii, colistin

Sažetak
Background: Acinetobacter baumannii is an emerging multidrug-resistant (MDR) nosocomial pathogen of the 21st century, often sensitive only to colistin. However, with increase in use, the resistance to colistin also slowly emerges. The aim of this study was to elucidate the underlying genetic mechanisms of colistin resistance in A. baumannii isolates from University Hospital Centre (UHC) Split and find a correlation between clinical data and development of resistance. Material/methods: Consecutive, non-duplicate carbapenem resistant A. baumannii isolates from patients treated in UHC Split in period from January 2014 to September 2016 were tested for colistin resistance by gradient test method according The European Committee on Antimicrobial Susceptibility Testing (EUCAST). In colistin-resistant isolates, the mechanisms of resistance were investigated by polymerase chain reaction (PCR) using primers for lpxA, lpxC and lpxD genes involved in lipid A biosynthesis pathway, followed by sequencing of PCR products and subsequent analyses. Additionally, the presence of blaOXA genes encoding OXA-type carbapenemases was also investigated by multiplex PCR and sequencing. Clinical data were collected from medical records. Results: We tested a total of 1430 A. baumannii isolates and four showed resistance to colistin. While in three of them minimal inhibitory concentrations (MICs) were slightly above the limit value displaying heteroresistance, one isolate had a high-level colistin resistance with MIC >256 mg/L. Only this isolate showed a presence of mutations in lpxC gene resulting in amino-acid changes that possibly introduced modifications in the lipid A component of lipopolysaccharide (LPS) and the initial binding target of polymixin antibiotics. The same isolate also carried the gene for OXA-23-like carbapenemase that showed 100% sequence identity with already described clinical isolates. All patients with resistant isolates were receiving extended colistin therapy. The patient with high-level resistant isolate underwent coronary artery bypass graft (CABG) surgery and aortic valve replacement (AVR), and developed mediastinitis. In his case, the omental flap coverage for reconstruction of sternotomy wound was required, but unfortunately he passed away. Conclusions: Mutations in three genes of the lipid A biosynthesis pathway are known to be connected with fast development of high-level resistance to colistin. In this study, mutations in lpxC gene of A. baumannii strain with high-level colistin resistance were identified. Amino-acid changes introduced by mutations could alter the lipid A protein and be responsible for reduced or abolished interaction with polymixins. However, other mechanisms of colistin resistance could also be involved, considering a very high level of resistance. Colistin-resistant A. baumannii occurred in patients who were receiving prolonged colistin treatment. Such patients should be carefully monitored for early detection of colistin resistance. Furthermore, the presence of OXA-23-like in carbapenem resistant isolate of A. baumannii from UHC Split is reported for the first time.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA