Pregled bibliografske jedinice broj: 87053
Novel polyketide products from manipulation of the oxytetracycline (otc) pathway of Streptomyces rimosus.
Novel polyketide products from manipulation of the oxytetracycline (otc) pathway of Streptomyces rimosus. // Program and Abstracts / Dainius, Donna (ur.).
Vancouver: University of British Columbia, 2001. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 87053 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Novel polyketide products from manipulation of the oxytetracycline (otc) pathway of Streptomyces rimosus.
Autori
Perić, Nataša ; Borovička, Branko ; Bago, Anita ; Hranueli, Daslav ; Hunter, S. Iain
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Program and Abstracts
/ Dainius, Donna - Vancouver : University of British Columbia, 2001
Skup
12th International Symposium on the Biology of Actinomycetes
Mjesto i datum
Vancouver, Kanada, 05.08.2001. - 09.08.2001
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Streptomyces rimosus; oxytetracycline; otcC gene; gene disruption; gene replacement
Sažetak
OtcC encodes a hydroxylase that acts at the 6-position of the tetracyclic nucleus, after it has beenformed. OtcC is located at the edge of the otc cluster, distant (and transcribed separately) from the genes for biosynthesis of the polyketide backbone. Disruption of otcC resulted, surprisingly, in a series of incorrectly-folded shorter polyketides (we had expected the tetracyclic structure without a 6-hydroxyl, which has been characterized previously in mutants studied in the 1960's). When a catalytically-inactive mutant version of otcC was used to replace this disrupted copy, the ability to make tetracyclic structures was restored. We hypothesize that OtcC is required as a structural component at the quaternary level to ensure fidelity of the polyketide synthase complex in generating the tetracyclic nucleus.
Izvorni jezik
Engleski
Znanstvena područja
Prehrambena tehnologija
