Naslov
Dynamics of adhesion and spreading of single cell at a charged interface

Autori
Ivošević DeNardis, Nadica ; Pečar Ilić, Jadranka ; Ružić, Ivica ; Pletikapić, Galja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Building bridges in biophysics Arbre Mobieu

Mjesto i datum
Porto, Portugal, 22.03.2017. - 24.03.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Cell adhesion ; dynamics ; plasma membrane vesicels

Sažetak
The single cell-electrode interaction is relevant to fundamental biological processes involving cell adhesion, cell fusion, cell activity, and membrane response to environmental stress. Cell surface properties play important role for the adhesion study. Model unicellular green algae Dunaliella tertiolecta is known as „naked cell“ since it poses only cell membrane. Adhesion and spreading of a cell at a charged interface cause double layer charge displacement which is measured as a well defined amperometric signal in millisecond time scale. The signal denotes establishment of intimate membrane contact at the interface with subsequent cell spreading, followed by cell breakdown in a few milliseconds. Dynamics of the cell adhesion at the charged interface has been examined by application of the reaction kinetics model on the amperometric signal.1 Model enables to decouple and identify temporal evolution of the individual states. Results suggest that the major structural disruption of the cell membrane, collapse of cytoskeleton and leakage of intracellular material appear 0.1 ms after initial cell-electrode contact. Kinetics of spreading of organic film at the interface to its maximal extent is being considered as the rate determining step, which could be consequence of the attenuated effect of potential at the modified interface, stronger intermolecular interactions and reorganization of molecules in the film. A simple system of an isolated plasma membrane vesicle without internal content has been developed and characterized by atomic force microscopy (AFM). Adhesion kinetics of the algal cell is slower than that of its plasma membrane vesicle. Our findings offer insight into the mechanism of cell adhesion and spreading at the charged interfaces. Reference 1. N. Ivošević DeNardis, J. Pečar Ilić, I. Ružić, G. Pletikapić, Cell adhesion and spreading at a charged interface: Insight into the mechanism using surface techniques and mathematical modelling. Electrochim. Acta 2015, 176, 743-754.

Izvorni jezik
Engleski

Znanstvena područja
Geologija

POVEZANOST RADA