Naslov
Electrochemical Probing of Estradiol Antioxidant Capacity

Autori
Haramustek, Ivana ; Martinez, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
YISAC 2010, Book of Abstracts / Ugo, Paolo - Venecija : Universita Ca' Foscari Venezia, 2010, 17-17

Skup
YISAC 2010

Mjesto i datum
Venecija, Italija, 29.06.2010. - 01.07.2010

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antioxidant ; estardiol ; cyclic voltammetry

Sažetak
Electrochemistry is a useful technique for studying redox reactions, and the literature is replete with electrochemical methods applied to biology. In particular, cyclic voltammetry (CV) has been conveniently used and validated for the quantification of in vitro low molecular weight antioxidant (LMWA) capacity of biological fluids, tissue homogenates, various foods and beverages and of LMWA capacity in vivo [1] and references within]. CV provides biological oxidation potential which relates to the nature of the molecules and peak currents and areas of the anodic current waves that relate to the concentration of the antioxidant molecules. A few papers describe the application of CV to measure estradiol (E2) concentration and antioxidant properties [2-5]. Antioxidant capacity of E2 in plasma and different tissues is of particular interest due to the role of its natural loss in the etiology of many diseases [6] that are believed to be caused and/or aggravated by oxidative stress. In the present study, we investigate new experimental strategies for measuring the antioxidant capacity of E2 by cyclic voltammetry at electrodes of various sizes, made of various materials. Measurements were done in phosphate buffer saline solutions of E2, in the range of physiological concentrations and in blood plasma. The fabricated electrodes were shown to be active for both, the surface adsorption of E2 and its electrocatalytic oxidation, and can be used for determination of estrogens in real samples. The investigation was also aimed towards downsizing of the electrodes to micrometric [7] and nanometric [8] dimensions that would enable antioxidant probing of individual cells and of the local cell environment, respectively. References 1. Kohen R, Nyska A, Toxicol. Pathol., 30, 620 (2002) 2. Hea Q, Yuana S, Chena C, Hua S, Mater. Sci. Eng. C, 23, 621 (2003) 3. Ngundi MM, Sadik OA, Yamaguchi T, Suye S, Electrochem. Commun., 5, 61 (2003) 4. Xiangqin L, Yongxin L, Biosens. Bioelectron., 22, 253 (2006) 5. Jin G-P, Lin X-Q, Electrochim. Acta, 50, 3556 (2005) 6. Unfer TC, Conterato GMM, da Silva JCN, Duarte MMMF, Emanuelli T, Clin. Chim. Acta, 369, 73 (2006) 7. Amatore C, Arbault S, Melo DC, Tapsoba I, Verchier Y, J. Electroanal. Chem., 34, 615 (2008) 8. Sun P, Laforge FO, Abeyweera TP, Rotenberg SA, Carpino J, Mirkin MV, PNAS 105, 443 (2008)

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo

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