Naslov
Flow cytometer monitoring of Bnip3- and Bnip3L/Nix- dependent mitophagy

Autori
Šprung, Matilda ; Dikić, Ivan ; Novak, Ivana

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Mitophagy: Methods and Protocols

Urednik/ci
Hattori, Nobutaka ; Saiki, Shinji

Izdavač
Humana Press

Grad
New York (NY)

Godina
2018

Raspon stranica
105-110

ISBN
978-1-4939-7749-9

ISSN
1064-3745

Ključne riječi
Bnip3L/Nix ; Flow cytometry ; MitoTracker ; Mitophagy ; Mitophagy receptor

Sažetak
Mitochondria are organelles with numerous vital roles in cellular metabolism. Impaired or damaged mitochondria are degraded in autophagolysosomes in a process known as mitophagy. Given the fundamental role of mitophagy in maintenance of cellular homeostasis, methods and techniques with which to study this process are constantly evolving and emerging. So far, mitophagy flux was mostly monitored using fluorescently labeled LC3 protein on autophagosomal membrane and any of the labeled outer mitochondrial membrane proteins. However, this method is labor intensive, time consuming, and difficult to quantitatively validate due to the rapid mitochondrial turnover. Here, we describe a flow cytometry as a novel and promising quantitative method to monitor Bnip3- and Bnip3L/Nix-mediated mitophagy.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

Napomena
Book Series: Methods in Molecular Biology

POVEZANOST RADA