Naslov
Viral Diversity in Autochthonous Croatian Grapevine Cultivars

Autori
Vončina, Darko ; Al Rwahnih, Maher ; Rowhani, Adib ; Gouran, Mona ; Almeida, Rodrigo P.P.

Izvornik
Plant disease (0191-2917) 101 (2017), 7; 1230-1235

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
grapevine, virus, variants, RT-qPCR

Sažetak
A survey was conducted on nine autochthonous grapevine cultivars grown along the Croatian coastal region. In total forty-eight vines (44 from germplasm collection, 4 from vineyards) originating from 23 sites were tested for 31 viruses using molecular methods. Results revealed high infection rates with Grapevine leafroll-associated virus 3 (GLRaV-3) and Grapevine virus A (GVA, both 91.7%), Grapevine fleck virus (GFkV, 87.5%) and Grapevine rupestris stem pitting-associated virus (GRSPaV, 83.3%). Other detected viruses were: Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated viruses 1, 2 and 4 (GLRaV- 1, GLRaV-2, GLRaV-4), Grapevine viruses B, D, F (GVB, GVD, GVF), Grapevine red globe virus (GRGV), Grapevine vein feathering virus (GVFV), Grapevine Syrah virus-1 (GSyV-1) and Grapevine Pinot gris virus (GPGV). No virus-free vine was found. Mixed infections were determined in all vines, the number of viruses in a single vine ranged from three to nine. GLRaV-3 variant typing confirmed presence of group I, II and III. Four vines with leaf deformation and mottling were positive for GPGV. Seven viruses (GLRaV-4 like group, GVD, GVE, GVF, GRGV, GSyV- 1 and GVFV) were detected for the first time in Croatia. This survey confirmed the deteriorated sanitary status of autochthonous Croatian grapevine cultivars.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

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