Naslov
Neuronal differentiation in the early human retinogenesis

Autori
Rančić, Anita ; Filipović, Natalija ; Marin Lovrić, Josipa ; Mardešić, Snježana ; Saraga-Babić, Mirna ; Vukojević, Katarina

Izvornik
Acta Histochemica (0065-1281) 119 (2017), 3; 264-272

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Eye development ; NF200 ; TH ; Chat

Sažetak
Aim: Our study investigates the differentiation of retinal stem cells towards different neuronal subtypes during the critical period of human eye development. Methods: Expression of the neuronal marker neurofilament 200 (NF200), tyrosine hydroxilase (TH) and choline acetyltransferase (ChAT) was seen by immunofluorescence in the 5th–12th – week stage of development in the human eye. Data was analysed by Mann– Whitney, Kruskal–Wallis and Dunn’s post hoc tests. Results: NF200, TH and ChAT cells appeared in the 5th/6th week and gradually increased during further development. The proportion of TH positive areas were distributed similarly to NF200, with a higher proportion in the outer neuroblastic layer. The proportion of a ChAT positive surface was highest in the 5th/6th – week whilst from the 7th week onwards, its proportion became higher in the optic nerve and inner neuroblastic layers than in the outer layer, where a decrease of ChAT positive areas were seen. Conclusions: Our study indicates a high differentiation potential of early retinal cells, which decreased with the advancement of development. The observed great variety of retinal phenotypic expressions results from a large scale of influences, taking place at different developmental stages.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA