Naslov
Comparative Analysis of Three Different STR Multiplex System Approaches In Fingerprint DNA Analysis

Autori
Causevic-Ramosevic A., Kovacevic L., Buljugic D., Dzehverovic M., Cakar J., Marjanovic D.

Izvornik
HealthMed (1840-2291) 6 (2012), 10; 3534-3539

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, ostalo

Ključne riječi
STR, Fingerprint, DNA

Sažetak
Aim: The aim of this study was to report on the efficacy of using miniSTR and STR markers in possible analysis of so called touch DNA samples which is representing "Low Copy Number" (LCN) DNA profiling. Methods: The study included 30 personal fingerprint samples (by the standard collection methods) and 30 buccal swab samples (referent samples). The samples were processed using 3 commercial kits (PowerPlex® S5, PowerPlex® ESI 17 and PowerPlex® 16). PCR amplification was carried out in PE GeneAmp 9700, PCR System Thermal Cycler. Detection of the amplificated products was performed on ABI PRISM 310 Genetic analyser (ABI, Foster City, CA), while RT-PCR DNA quantification was performed using Quantifiler DNA Identification kit. Results: Minimal concentrations of nuclear DNA were registered in 8 (27%) of 30 fingerprints, while they were undetectable in the other 22 (73%) of 30 fingerprints. Concentrations of detected isolated DNA ranged from 9, 33 × 10-3 pg/μl to 1, 41 × 10-2 pg/μl. Conclusion: Results of this study confirmed the presence of artificial alleles after use of all of the three commercial kits (PowerPlex® S5, PowerPlex® 16 and PowerPlex® ESI 17). This suggests possible contamination during sample collection, but also appearance of allele drop in and drop out. Allele drop in and allele drop out are expected while performing analysis of small amounts of DNA, especially during PCR protocol optimization. Consequently, further studies should investigate the optimization of all steps of DNA analysis procedures.

Izvorni jezik
Engleski

POVEZANOST RADA