Naslov
Genotoxicity of cadmium chloride in human lymphocytes evaluated by the comet assay and cytogenetic tests

Autori
Rozgaj, Ružica ; Kašuba, Vilena ; Fučić, Aleksandra

Izvornik
Journal of trace elements in medicine and biology (0946-672X) 16 (2002), 3; 187-193

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cadmium chloride; genotoxicity; comet assay; micronucleus test; chromosome aberrations; sister chromatid exchanges

Sažetak
Peripheral blood lymphocytes were tested in vitro for genotoxic effects of cadmium chloride. Whole blood samples of four healthy , non-smoking subjects were preincubated with CdCl2 in concentrations of 10-4 and 5x10-5 mol/L for three hours before the cells were assessed for DNA-damage using the single cell alkaline gel electrophoresis assay (comet assay) or cultivated for chromosomal aberrations(CA), sister chromatid exchanges (SCE) and the micoronucleus (MN) test. The comet assay showed notable interindividual differences. The result of the cytogenetic tests showed an increase in the frequency of CA, MN and SCE with CdCl2 in the treated cultures, yet none was able to show a correlation between concentration of cadmium chloride and the frequency of damages. The MN slides were stained with Giemsa and with DNA fluorochrome 4, 6-diamino-2-phenylindole(DAPI). The frequency of MN in slides stained with DAPI was significantly higher than in those stained with Giemsa, which might be due to an underestimation of small micornuclei in Giemsa-stained slides

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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