Naslov
Catechol removal from aqueous media using laccase immobilized in different macro- and microreactor systems

Autori
Jurinjak Tušek, Ana ; Šalić, Anita ; Zelić, Bruno

Izvornik
Applied biochemistry and biotechnology (0273-2289) 182 (2017), 4; 1575-1590

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
laccase ; immobilization ; oxidation of catechol ; microreactor

Sažetak
Laccase belongs to the group of enzymes that are capable to catalyse the oxidation of phenols. Since the water is only by-product in laccase catalyse phenol oxidations, it is ideally ‘green’ enzyme with many possible applications in different industrial processes. To make the oxidation process more sustainable in terms of biocatalyst consumption immobilization of the enzyme is implemented in to the processes. Additionally, when developing a process, choice of a reactor type plays a significant role in the total outcome. In this study use of immobilized laccase from Trametes versicolor for biocatalytic catechol oxidation was explored. Two different methods of immobilization were performed and compared using five different reactor types. In order to compare different systems used for catechol oxidation biocatalyst turnover number and turnover frequency were calculated. With low consumption of the enzyme and good efficiency, obtained results go in favour of microreactors with enzyme covalently immobilized on the microchannel surface.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA