Pregled bibliografske jedinice broj: 856149
Analysis of N-Glycosylation of Total Membrane Proteins
Analysis of N-Glycosylation of Total Membrane Proteins // High-Throughput Glycomics and Glycoproteomics / Lauc, Gordan ; Manfred, Wuhrer (ur.).
New York (NY): Springer, 2017. str. 197-205
CROSBI ID: 856149 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Analysis of N-Glycosylation of Total Membrane Proteins
Autori
Pavić, Tamara ; Gornik, Olga
Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni
Knjiga
High-Throughput Glycomics and Glycoproteomics
Urednik/ci
Lauc, Gordan ; Manfred, Wuhrer
Izdavač
Springer
Grad
New York (NY)
Godina
2017
Raspon stranica
197-205
ISBN
978-1-4939-6491-8
Ključne riječi
N-Glycans ; Membrane proteins ; Cloud point extraction ; Triton X-114 ; Protein enrichment ; HILIC-UPLC
Sažetak
Glycosylation of membrane proteins plays a crucial role in various physiological events, including intercellular recognition and intermolecular interactions on the cell surface (Gornik et al., Biochim Biophys Acta 1820:1318– 1326, 2012). To study composition and function of N-glycans on membrane proteins one has to have an efficient and reproducible analytical method, which includes protein extraction and analysis of glycans. In this chapter we provide an analytical approach that includes cloud- point extraction (CPE) of total membrane proteins with the non-ionic detergent Triton X- 114 and subsequent analysis of their N-glycans using hydrophilic interaction liquid chromatography (HILIC)–UPLC/HPLC. The protocol presented here can be used for parallel analysis of both membrane and intracellular proteins.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb
