Naslov
Macro- and microelement in rat liver, kidney, and brain tissues: sex differences and effect of blood removal by perfusion in vivo

Autori
Orct, Tatjana ; Jurasović, Jasna ; Micek, Vedran ; Karaica, Dean ; Sabolić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 5th Croatian Congress of Toxicology with International Participation (CROTOX 2016) ; u: Arhiv za higijenu rada i toksikologiju 67 (2016) (S1) / Kopjar, Nevenka - , 2016, 50-50

Skup
5th Croatian Congress of Toxicology with International Participation (CROTOX 2016)

Mjesto i datum
Poreč, Hrvatska, 09.10.2016. - 12.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
gender differences ; ICP-MS multielement analysis ; perfusion ; rat organs

Sažetak
The concentrations of macro- and microelements in tissues could be sex-related and affected by blood retained in dissected organs. To test these hypotheses, in 3-mo old female and male rats, the concentrations of various elements were measured in the blood-containing (nonperfused) and blood- free (perfused in vivo with an elements-free buffer) liver, kidney, and brain (N=5 in each group). In these organs, 6 macroelements and 15 microelements were determined by ICP-MS. In non- perfused organs, female-dominant differences were observed for Fe, Se, and I in the liver ; Fe, Co, Zn, Cd, and Li in the kidney ; and As and Li in the brain. In blood-free organs, similar differences were detected for: Mg, K, Fe, Mn, Cu, Se, I, and Cd in the liver ; Fe, Co, and Li in the kidney ; and none in the brain. In females, the perfused liver, kidney, and brain had significantly lower concentrations of 4 (Na, Ca, As, B), 5 (Na, K, I, As, Li), and 2 (Fe, As) elements, and higher concentrations of 10 (Mg, P, S, K, Mn, Cu, Zn, Cd, Hg, Li), 4 (Co, Pb, Cd, Hg), and 7 (Mg, S, K, Zn, Se, I, Li) elements. In males, perfusion caused lower concentrations of 4 (Na, Ca, As, B), 7 (Na, Ca, Fe, I, As, Hg, Li), and 2 (Fe, As), and higher concentrations of 1 (Li), 1 (Zn), and 7 (Mg, P, S, K, Zn, I, Li) elements in the liver, kidney, and brain, respectively. Therefore, residual blood in organs can significantly influence the tissue concentrations of various elements and their sex- dependency. This work was financially supported by Project No. 1481 Aging-related Expression of Membrane Transporters in Rat (AGEMETAR), funded by the Croatian Science Foundation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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