Pregled bibliografske jedinice broj: 854966
Razvoj metode za određivanje onečišćenja u nepafenak djelatnoj farmaceutskoj tvari kromatografijom superkritičnih fluida
Razvoj metode za određivanje onečišćenja u nepafenak djelatnoj farmaceutskoj tvari kromatografijom superkritičnih fluida // XVI. Ruzickini dani “DANAS ZNANOST – SUTRA INDUSTRIJA"
Vukovar, Hrvatska, 2016. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 854966 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Razvoj metode za određivanje onečišćenja u nepafenak djelatnoj farmaceutskoj tvari kromatografijom superkritičnih fluida
(Developlment of method for determination of impurities in nepafenac by supercritical fluid chromatography)
Autori
Mislav Runje ; Olga Malev ; Irena Raič ; Igor Avdejev ; Sandra Babić
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
XVI. Ruzickini dani “DANAS ZNANOST – SUTRA INDUSTRIJA"
Mjesto i datum
Vukovar, Hrvatska, 21.09.2016. - 23.09.2016
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
nepafenak; kromatografija supekritičnih fluida; onečišćenja
(nepafenac; supercritical fluid chromatography; impurities)
Sažetak
Nepafenac is chemically described as 2-amino-3-benzoylbenzeneacetamide manufactured as a 0.1% suspension for topical ophthalmic use [1] and it belongs to a group of nonsteroidal anti-inflammatory drugs (NSAID). A novel simple, fast and efficient supercritical fluid chromatography (SFC) method was developed and compared with reversed-phase liquid chromatography (RPLC) method for the separation and determination of impurities in Nepafenc [2-3]. Overall satisfactory resolutions and peak shapes for Nepafenac, 2-aminobenzophenone, Cl-thionepafenac, Thionepafenac, Cl-Nepafenac, Hydroxy Nepafenac and Cyclic Nepafenac were obtained by optimization of the chromatography system. With the gradient elution of mobile phase, all of the impurities and the active ingredient were separated within 6 min. Taking full advantage of features of SFC (such as particular selectivity, non-sloping baseline in gradient elution, and without solvent injection effects), the method was successfully used for determination of impurities in Nepafenac. More impurity peaks were detected, better resolution was achieved and much less analysis time was needed in comparison with conventional RPLC methods. The developed SFC method is validated with respect to specificity, LOD, LOQ, linearity, precision, accuracy and robustness.
Izvorni jezik
Engleski
Znanstvena područja
Farmacija
