Naslov
Plomin bay sediments are not mutagenic in modified preincubation Ames assay

Autori
Franekić Čolić, Jasna ; Durgo, Ksenija ; Oreščanin, Višnja ; Valković, Vladivoj

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Toxicology letters / - Amsterdam : Elsevier, 2001, 109-109

Skup
Abstracts of EUROTOX 2001

Mjesto i datum
Istanbul, Turska, 13.10.2001. - 16.10.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Plomin; Water sediment; mutagenic assay; Salmonella typhimurium

Sažetak
Disposal of the dredged material fromthe harbour to the sea bottom represents a route through which many contaminants could be transported towards the open sea and enter to the food chain. Inthis study, five sediment samples were taken from different depths and locations within Plomin bay ( Istria, Peninsula, Northern Adriatic sea, Croatia). Water sediment extract were prepared and analysed for chemical and physical properties, and subjeted to cytotoxic and mutagenic investigations. We used two strains of salmonella typhimurium, TA98 (determination of frameshift mutation) and TA100 (detection of base pair substitution) as a test systems. The insensivity of bacteria to heavy metals cytotoxicity and mutagenicity was avoided by changing classical Ames assay with the modified preincubation assay. for mutagenic assay, sediment extracxts and bacterial strains were preincubated for 24h on 37^C (with and withought metabolic activation) and incorporated minimal medium. After 72h of incubation the numberof revertant colonies was counted. Results showed thatconcentrations of heavy metals measured in extracts represent exchangeable fractionand are 500-1000 times smaller than the content of the same elements in solid mud. Nine elements were determined in extracts: V, Cr, Mn, Fe, Ni, Cu, Zn, As and Pb. None of sediment sample extracts were cytotoxic or mutagenic for Salmonella typhimurium TA98 nor TA100 in presence/absence of metabolic activation.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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