Naslov
Cytotoxic and prooxidative effect of ginger extract on human laryngeal and lung carcinoma cell lines

Autori
Bušić, Arijana ; Durgo, Ksenija ; Komes, Draženka ; Vojvodić, Aleksandra ; Belščak-Cvitanović, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of International Conference 16th Ružička days, "Today science-tomorrow industry" / Jukić, Ante - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2016, 70-70

ISBN
978-953-6894-58-1

Skup
International Conference 16th Ružička days, "Today science-tomorrow industry"

Mjesto i datum
Vukovar, Hrvatska, 21.09.2016. - 23.09.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cytotoxicity ; ginger ; laryngeal and lung carcinoma cell lines ; lipid peroxidation ; prooxidative effect

Sažetak
Nowadays, cancer has become a leading cause of fatal diseases worldwide. Recently, the emphasis is dedicated on investigation of natural, alternative remedies in treatment of cancer cell lines, where plant extracts, due to their rich bioactive composition and high biological activity may play big role. In this study, biological effect of aqueous ginger (Zingiber officinale) extract (GE) on the human larynx cancer cell line (Hep2) and lung carcinoma cells (A549) was evaluated. Cytotoxic, prooxidative effect and lipid peroxidation were examined in cancer cell lines exposed to different concentrations of GE for 2 hours. Cell survival was determined by Neutral red test, while 2’, 7’ dichlorofluoresceine diacetate (DCFDA) method was used for determination of free radicals. Lipid peroxidation was evaluated by measuring concentration of generated malondialdehides. GE showed significant decrease in cell survival for Hep2 and A549 in the highest concentration of 0.25 g/ml, while lower concentrations (0.05 and 0.125 g/ml) did not show cytotoxic effect. GE did not exhibit prooxidative effect, contrary, antioxidative effect of all concentrations on both cell lines was observed, where by increasing the concentration, free radical induction was decreased. Moreover, GE did not reveal effect on cell lipids. Obtained results confirmed markable health benefits of GE and justified its wide application in various pharmaceutical and food products.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

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