Naslov
Polymorphisms of pon1 gene in patients with premalignant lesion of the cervics

Autori
Stojić, Ana Marija ; Grdić Rajković, Marija ; Ćelap, Ivana ; Hulina, Andrea ; Eljuga, Damir ; Butorac, Dražan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Libri oncologici / - , 2016, 50-50

Skup
Fourth Meeting of the Croatian Association for Cancer Research with International Participation

Mjesto i datum
Zagreb, Hrvatska, 03.11.2016. - 04.11.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
paraoxonase; polymorphisms; premalignant lesions of the cervix

Sažetak
Increased lipid peroxidation and changes in antioxidative system in patients with premalignant lesions of the cervix indicates the importance of oxidative stress in early phase of carcinogenesis. Paraoxonase 1 (PON1) is a calcium-dependent esterase, synthesized in the liver and secreted into the plasma where it is associated with HDL. PON1 possess antioxidant/antiatherogenic activity, and it removes carcinogenic radicals of lipid peroxidation in human body. Polymorphisms in coding (Q192R and L55M) and promoter (-108 C>T) regions of pon1 gene affect PON1 activity. We carried out this study in order to determine frequencies of Q192R, L55M and -108C>T polymorphisms in patients with premalignant lesions of the cervics. Study includeded 65 womens [37 (29-42) years] with a biopsy confirmed diagnosis of cervical intraepitelial neoplasia (CIN). CIN1 had 18 patients, 23 had CIN2 and 24 had CIN3. 109 healty volonters [38 (32-46) years] without any cervical or other disease were enroled in the stady as a control group. DNA was isolated from whole blood and polymorphisms were determined by PCR-RFLP procedure. The analysis of pon1 gene polymorphisms in patient group showed following frequencies: 51 % QQ, 44 % QR, and 5 % RR for Q192R genotype ; 43 % LL, 43% LM, and 14 % MM for L55M genotype ; 35 % CC, 42 % CT, and 23 % TT for -108 C>T genotype. In the control group we found rather similar frequencies: 59 % QQ, 33 % QR, 8 % RR for Q192R genotype ; 40 % LL, 42 % LM, and 18 % MM for L55M genotype ; 30 % CC, 45 % CT, and 25 % TT for -108 C>T genotype. No significant differences was found concerning examined polymorphisms between patients and healthy volunteers (Q192R, P=0.258 ; L55M, P=0.728 ; -108C>T, P=0.784). Our results suggest that Q192R, L55M and -108C>T polymorphisms of pon1 gene is not a potential risk factors for cervical intraepithelial neoplasia. However this finding must be confirmed by study conducted on bigger sample size.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA