Naslov
Izolacija međuprodukta sinteze bočnih lanaca statina
(Intermediate isolation in the synthesis of statin side chains)

Autori
Česnik, Morana ; Švarc, Anera ; Sudar, Martina ; Vrsalović Presečki, Ana ; Vasić-Rački, Đurđa ; Findrik Blažević, Zvjezdana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XI. Susret mladih kemijskih inženjera, Knjiga sažetaka / Gordana Matijašić - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2016, 37-37

ISBN
978-953-6894-55-0

Skup
XI. Susret mladih kemijskih inženjera

Mjesto i datum
Zagreb, Hrvatska, 18.02.2016. - 19.02.2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
biokataliza ; statini ; enzimi ; aldolaza
(biocatalysis ; statins ; enzymes ; aldolase)

Sažetak
2-Deoxyribose-5-phosphate aldolase (DERA, EC 4.1.2.4) belongs to the group of acetaldehyde dependent aldolases and is the only one capable of condensing two aldehydes into a new aldehyde product. By using inexpensive, achiral chloroacetaldehyde and acetaldehyde as substrates, DERA catalyzes the sequential aldol condensation that produces polyhydroxy aldehyde precursor to statin side chains. Statins are one of the most successful stories of modern medicinal chemistry due to their cholesterol- lowering capabilities. The synthesis of the statin drug intermediate from acetaldehyde and chloroacetaldehyde catalyzed by DERA was performed in a fed-batch reactor. The obtained aldol product was isolated from the reaction mixture and purified. Isolation process involved several extraction steps and reverse phase preparative LC purification methods according to the literature. Resulting intermediate purity was specified by GC analysis. The isolation process was monitored and described in detail in this work. Also, some important conclusions were drawn that should improve the procedure in the future.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

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