Naslov
In vivo micronucleus assay-control values for children of 0-7 years age
(In vivo micronucleus assay-control values for children of 0.7 years age)

Autori
Fučić, Aleksandra ; Lasan, Ružica ; Hitrec, Vlasta

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Environmental and Molecular Mutagensis / Heflich r.H. - Hoboken (NJ) : Wiley-Liss, 2001

Skup
Conference of Environmental Mutagen Society: A Science Odyssey

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 16.03.2001. - 21.03.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
in vivo micronucleus assay; children

Sažetak
The development of new biomonitoring methods for evaluation of health risk due to environmental contamination or parental exposire redefines the child's position. The child is no longer a small human being but a unique entity with specific response to environment especially in first few years of life. Small blood sample and the quickness of the procedure, as it does not rquire cell culture, makes in vivo micronucleus assay elegant method for biomonitoring of children. The in vivo micronucleus assay is preferentially applied in genotoxicological experiments with rodents (Hayashi et al 1997). The results of biomonitoring of human population using this method are limited. We analysed 30 chiildren aged from 0-7 years. the results show that the average frequency of micronuclei in peripheral blood is 0,21%. There was no significant differences in micronuclei frequency between girls and boys. These agrees with published data for adult population (Xue et al., 1992). Small interindividual differences, low cost of method and quick results mark this method as suitable for biomonitoring of genome damage in children after exposure to clastogens and aneugens via environmental pollution. Small blood samples amkes the method applicable even in early postnatal period. Further research should give data on sensitivity of method and stability of this biomarker.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA